Paris Saint-Germain announced on Friday that Lionel Messi will miss the team's trip to Reims due to a calf injury.
After PSG drew away to Benfica in the Champions League on Wednesday in Lisbon, coach Christophe Galtier claimed the Argentine star was "fatigued."
With nine minutes remaining in the 1-1 tie, Messi, 35, who had scored PSG's goal, was substituted by Pablo Sarabia.
“Leo will be rested. He will resume running on Sunday and will train on Monday before the return match against Benfica,” said Galtier.
Galtier and PSG face nine games before breaking for the World Cup on November 13.
Messi’s Argentina kick off their campaign in Qatar against Saudi Arabia on November 22.
It will be Messi’s fifth World Cup and he said this week it will definitely be his last.
“It’s my last World Cup, surely. I feel good physically,” he told ESPN Argentina on Thursday.
“It was essential to get to where I am, with a good state of mind and a lot of hope.”
The PSG medical statement said Messi was suffering “discomfort in the calf”.
It added that Kylian Mbappe is recovering from a sore throat and “trained in the gym” but he is a doubt for the match in Reims.
PSG host Benfica on Tuesday and the following Sunday face bitter Ligue 1 rivals Marseille, who are just two points behind in the Ligue 1 table going into this weekend’s matches.